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which one will come first in hplc

http://www.chromforum.org/viewtopic.php?t=12829

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which one will come first in hplc

Posted: Mon Apr 26, 2010 6:54 pm
by mk12
i have a aglycone flavanoid which is comming at 7.2 min and its suspected aminodeoxy hexose derivative is comming at 8.3 min. is it right order in HPLC? i was thinking glycoside will elute earlier then aglycone.


Chormatographic conditions are

C18 UPLC column 1.9 micron
Gradient: 0-100% ACN in 10 min.

please help

Posted: Tue Apr 27, 2010 3:16 pm
by orcicdejan
Elution order is (in my experience):

hexuronosides < hexosides, malonylhexosides < acetylhexosides, aglycones

So, I would guess your aminodeoxyhexoside will also elute before the algycone.
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