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Column test mix for LCMS Ion Trap

Posted: Mon Jan 10, 2005 3:32 am
by Drew
I am currently routine using a C18 method with LCMS Ion Trap (ESI) to screen for my sample. The mobile phase is gradient . Mobile phase 1 is 0.1% TFA in water . The gradient is from 99% to 30% in 30minutes. I need to develop a column test mix containing void marker, neutral probe , acidic probe and basic probe. The Mw of my compounds range from 90 to 250. Please advice on the type of compounds which l can use

Thanks and Regards
Drew