Bisphenol A with CG/ECD

[Juan Pedro]

Hello everybody!

We want to quantify Bisphenol-A with GC-ECD, anyone could give me some information?

Thank you very much!

[jerole]

Correct me if I am wrong, but if you mean Electron Capture Detection that shouldn't work. Electrochemical Detectors are used in LC for Bisphenol A detection.

[AICMM]

You might be able to use pentafluorobenzyl bromide to derivitize the Bisphenol A and get a compound that should be detectable by ECD.

Best regards.

[PetrolEr]

Hello everybody!

We want to quantify Bisphenol-A with GC-ECD, anyone could give me some information?

Thank you very much!

Dear, J. Pedro,

First,
you must use PID (Photoionization Detector). The FID is similar good, but it's give in some case weaker signal like the PID (Why? Look the ionizaion energy of aromatic and aliphatic compounds!), but the FID has wider dynamic range.

Second,
Use DB-5 or DB5HT or similar column (5%diphenyl-95%dimethyl-polysiloxane or similar film coating). These columns are very good for analysis of the substituated phenols and quinolins in ideally case. Use appropriate makeup, carrier, etc. gases!

The ECD isn't the best choice. It is good for detectation of halogenated CH's.

[gpronger]

I have been looking at approaches to analysis of BPA for a few months, and there seems to be two main venues; 1) HPLC and 2) Derivitization and GC or GCMS. The former appears to be somewhat more commonplace, likely due to historic issues with GC of BPA. There are a lot of ways the derivitization is handled, sometimes with perfluoro or silyl functionalities. The perfluoro approach should give good response to an ECD. The silyl is common where the lab is going to GCMS SIM where the gain in AMU raises the quantitation ion, which generally decreases potential for interferences.

For us (Suburban Labs), we have been able to "tweak" EPA method 625 and have validated the method at 1PPB. The benefit for us is that for a water study we can report the BPA to a reasonable level of sensitivity while being able to report the typical full list of 625 compounds. Obviously, SIM would improve detection levels, but it is then more difficult to quantitate everything else.

Greg

[tangaloomaflyer]

I am also interested in developing a method for analysis of BPA and DEHP (Bis-ethylhexylphthalate) in plastic consumer products. I already have a GC-MS method that can resolve DEHP, but coming from an environmental background I'm not too sure about the extraction.

I presume a simple extraction into a polar solvent for DEHP and (maybe an acidified leach for the BPA?) but I would like to get hold of some sort of standardised plastic matrix containing a known amount of DEHP/BPA for a positive control and a DEHP/BPA free plastic for the blank. Are these sort of materials available?

thanks

[gpronger]

The CPSA has recently published the following procedure:

http://www.cpsc.gov/about/cpsia/CPSC-CH-C1001-09.3.pdf

It should have what you're looking for.

Greg

[ramsrestler]

The best technique for bis Phenol-A and the other phalates is actually using a Frontier Thermal Desorption/Pyrolyzer. On the lastest round robin testing...it received the only 100% score.

Check it out on www.frontier-lab.com

[gpronger]

The best technique is what will be accepted by the regulators. There are a lot of vendors with fairly well considered products, but until they receive regulatory approval, you need to be cautious before pursuing this avenue if your data will end up submitted for compliance purposes.

I looked over the Frontier website and could not find information related for approval for this application by the CPSC. I took a quick look at the CPSC and wasn't able to locate thermal desorption as an approved alternative.