Chromatography Forum

I used RP-MPLC (Water/ACN unbuffered) to purify a amino acid after ester hydrolysis (aeq. NaOH), the target compound was apparantly isolated as its sodium salt. Problem is, boss doesn't believe me and wants to see a paper discussing the posibility of sodium salt isolation after RP-purification. Any suggestions?

Hi

If I do not misunderstand you completly, your boss needs a crash course in basic organic chemistry

Seroiusly, you can search for articles like for example drug substances processes where a sodium salt is made in the last step of the syntheis.

Your LC clean up seems very much the same as a second recrystillization step in a process only smaller.

Typical breif flow: crude drug substance is dissolved in solvent, water/naoh is added. Crude Na-drug salt is crystillazied. Crude Na-drug salt is redissolved and then recrystillazied= Pure Na-drug.

A googled link with a few examples: http://www.patentstorm.us/patents/62071 ... ption.html

count_zero, do you think that the sodium salt of the amino acid comes out of the column? Why?
(MP is medium pressure?)

mmm

now I understand your boss question, I assumed you worked up your collected fraction.