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Clenbuterol by LC
Posted: Fri Apr 16, 2010 2:47 am
by thohry
Hi all,
Could I analyze clenbuterol by HPLC/UV (or fluorescence) . I have searched and there are many applications in LC/MS , not HPLC.
Thanks
Posted: Fri Apr 16, 2010 5:04 am
by bisnettrj2
Search PubMed.gov, enter 'clenbuterol hplc' as your search term.
Posted: Fri Apr 16, 2010 6:59 am
by thohry
Thank you very much.
Posted: Fri Apr 16, 2010 4:19 pm
by krickos
Hi
If you have difficulties you may want to widen search a bit. This drug belong to the same family as Salbutamol, bambuterol, terbutaline. Should be a bunch of articles related to those and some pharmacopiea methods aswell.
Should be a method in the eurpean pharmacopiea but can not confirm as I am not in office curently.
Posted: Tue Apr 20, 2010 10:14 am
by krickos
Here is the Ph Eur method:
Related substances. Liquid chromatography (2.2.29).
Test solution. Disperse 100.0 mg of the substance to be examined in the mobile phase and dilute to 50.0 ml with the mobile phase.
Reference solution (a). Dilute 0.1 ml of the test solution to 100.0 ml with water R.
Reference solution (b). Dissolve 5 mg of clenbuterol impurity B CRS in 10 ml of the mobile phase, add 2.5 ml of the test solution and dilute to 25.0 ml with the mobile phase.
Column:
— size: l = 0.125 m, Ø = 4 mm,
— stationary phase: end-capped octadecylsilyl silica gel for chromatography R (5 µm),
— temperature: 40 °C.
Mobile phase: mix 200 volumes of acetonitrile R, 200 volumes of methanol R and 600 volumes of a solution prepared as follows: dissolve 3.0 g of sodium decanesulphonate R and 5.0 g of potassium dihydrogen phosphate R in 900 ml of water R, adjust to pH 3.0 with dilute phosphoric acid R and dilute to 1000 ml with water R.
Flow rate: 0.5 ml/min.
Detection: spectrophotometer at 215 nm.
Injection: 5 µl.
Run time: 1.5 times the retention time of clenbuterol .
Retention time: clenbuterol = about 29 min.
System suitability: reference solution (b):
— resolution: minimum 4.0 between the peaks due to impurity B and clenbuterol.
Limits:
— impurities A, B, C, D, E, F: for each impurity, not more than the area of the principal peak in the chromatogram obtained with reference solution (a) (0.1 per cent),
— any other impurity: for each impurity, not more than the area of the principal peak in the chromatogram obtained with reference solution (a) (0.1 per cent),
— total: not more than twice the area of the principal peak in the chromatogram obtained with reference solution (a) (0.2 per cent),
— disregard limit: 0.5 times the area of the principal peak in the chromatogram obtained with reference solution (a) (0.05 per cent).