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Posted: Tue Apr 13, 2010 1:08 pm
by aalbre
might be a stupid question, but is it possible to prepare ammonium bicarbonate buffer @ pH 6.5? If so, how and is it stable?

Posted: Wed Apr 14, 2010 3:08 am
by tom jupille
The pKa of carbonic acid/bicarbonate is listed as 6.36, so you should be able to make a solution of ammonium bicarbonate and tweak the pH with a bit of ammonium hydroxide. It's not very stable when exposed to air, however, because the carbonic acid (H2CO3) goes away as CO2 over time. In a closed system (like inside a blood vessel) it can be stable.
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