LC-MS buffer compatibility
Posted: Mon Apr 12, 2010 8:38 am
Hi,
i came across some compounds (buffers) on the internet that are supposed to be MS compatible in connection with ion exchange chromatography (proteomics):
carbonic acid, formic acid, acetic acid, trifluoroacetic acid, propionic acid, propargylic acid, maleic acid, malonic acid, 2-methyl malonic acid, 2,2-dimethyl malonic acid, 2-ethyl malonic acid, and 2,2,-diethyl malonic acid, ammonium hydroxide, ammonium bicarbonate, methylamine, ethylamine, trimethylamine, triethylamine pyridine, methyl substituted pyridine, pyrazine, pyridazine and pyrimidine.
Are all of them really used in LC-MS either as pure substances or as their salt? and what is normally the upper limit for the concentration of such buffers so that the ion supression in the ion source is not too high?
i came across some compounds (buffers) on the internet that are supposed to be MS compatible in connection with ion exchange chromatography (proteomics):
carbonic acid, formic acid, acetic acid, trifluoroacetic acid, propionic acid, propargylic acid, maleic acid, malonic acid, 2-methyl malonic acid, 2,2-dimethyl malonic acid, 2-ethyl malonic acid, and 2,2,-diethyl malonic acid, ammonium hydroxide, ammonium bicarbonate, methylamine, ethylamine, trimethylamine, triethylamine pyridine, methyl substituted pyridine, pyrazine, pyridazine and pyrimidine.
Are all of them really used in LC-MS either as pure substances or as their salt? and what is normally the upper limit for the concentration of such buffers so that the ion supression in the ion source is not too high?