Chromatography Forum

Hellow,

How can analyzed these two compound on RP_HPLC I don't have Any HILIC or amino column.
I have symmetry shield or symmtery column or simple C-18 phase.
Can I analyzed these two on HPLC

Please suggest me

Praveen

There were a number of papers published in the 1980s on analysis of anions using ion-pair chromatography and "indirect uv" detection, but the technique has not proven to be very popular. Here's an example:
http://www3.interscience.wiley.com/jour ... 0/abstract

If you do a Google search on the keywords "ion pair" and "indirect UV" you will turn up more.

Chloride has certainly been done that way; I don't know about the cyanouric acid. In either case, it has not proven to be a very popular technique.

What is the sensitivity requirement for these two analytes?

I don't know how to retain and separate them using a RP column, but I know the method that can do it as described below:

Column: Acclaim Trinity P1 (Dionex)
Diemnsion: 3x50-mm
Mobile phase: MeCN/NH4OAc buffer (~10mM total), pH~5 v/v 60/40
Flow rate: 0.5 mL/min
Injection: 2 to 5 uL
Temperature: 30 C
Detection: Corona CAD or MS

Here is some information on this column: http://www.dionex.com/en-us/webdocs/707 ... 239-02.pdf

I want to analyze these two on RP_HPLC . Now what should I do?

I think cyanuric acid should be easily retained on your column. Just run a wide gradient starting at 3% organic and ramp up to 100% organic over 10 minutes or so and see where it comes out.

Tom's suggestion of ion pairing and indirect would probably work but it is kind of a pain which is why people don't do it much.

Here is a whacky idea that you could try for chloride. Put a layer of organic (maybe carbon tetrachloride or similar) on top. Add oxidizer like persulfate or perchlorate to turn the chloride into chlorine. Extract chlorine into organic. Inject organic and you may retain chlorine and measure by UV. I suspect but don't know that Cl2 will have a strong UV absorbption.

pr...., you are talking about two compounds in your original question, are you asking about chlorides of cyanuric acid or chloride ion (which I would not call a compound).
If you are really asking about chloride ion (why would one need to analyze such a combination?) maybe you can find a mobile phase pH where cyanuric is retained but chloride does ion exclusion.

swimming pool test kits?

cyanuric choride is unstable in water and methanol. it is easily converts into cyanuric acid. i tried it in RP chromatography, but i could not get consistent peaks. i