Hazy Solution

[syx]

dear all, we have got problem with hazy sample solution. filtration and centrifugation cannot produce clear solution. i have an idea about aggregation followed by filtration, but i do not know how to obtain the particle aggregates. does anyone help me? thanks in advance.

[Vlad Orlovsky]

Your hazy solution can be result of emulsion and not suspension. Try to add THF and see what happens.

[HW Mueller]

Ultrafiltration? But please be aware that you may have a severe complication here, regarding analysis of any (partially??) dissolved substances.

[Consumer Products Guy]

What have you tried for filtration? What is the solvent of your solution?

[Don_Hilton]

Cah you tell us what it is you are disolving? This may help come up with an answer.

[syx]

we want to determine B12 in multivitamin tablets. due to its small quantity we use small volume of solvent (pH 4-5 aqueous buffer) to obtain B12 in its working concentration that be able to quantity using LC. we found hazzy solution that could not be cleaned by centrifugation or filtration.

[Don_Hilton]

Look at the ingreedients list for items that are not soluable in water. That may give you some ideas. This would include both organic and inorganic compunds.

[danko]

Hi Syx,

If the multivitamin tablets contain some or all of the following vitamins A, D, E and K, which are fat- soluble the resultant “solutionâ€

[syx]

it does not contain lipid-soluble vitamins. the active ingredients are water soluble vitamins and minerals.

[Don_Hilton]

What are the inactive ingreedients?

[syx]

excipients that commonly be used in capsule formulations. some active ingredients are coated separately with unknown polymer(s) to avoid interaction with other substances in the formulation.

[Don_Hilton]

Well, the polymers are a good candidate for being the problem. Perhaps a solvent change will either make the polymer dissolve or agrregate?