Ibandronate sodium
Posted: Wed Mar 17, 2010 8:47 pm
Hi, I´m looking method analysis HPLC for Ibandronate sodium, someone help me?
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Ibandronate sodium
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Posted: Wed Mar 17, 2010 8:56 pm
Try Google on the keywords "Ibandronate sodium" and HPLC
Posted: Thu Mar 18, 2010 2:33 am
Here is a method for "dronic" acids:
http://www.sielc.com/compound_249.html
Also here is reference on validated method on similar compound (etidronic acid):
Direct stability-indicating method development and validation for analysis of etidronate disodium using a mixed-mode column and charged aerosol detector. Liu, X.K., Fang, J.B., Cauchon, N., Zhou, P. Journal of Pharmaceutical and Biomedical Analysis , Vol. 46, No. 4, pages 639-644 (2008)
Contact me if you have questions.
http://www.sielc.com/compound_249.html
Also here is reference on validated method on similar compound (etidronic acid):
Direct stability-indicating method development and validation for analysis of etidronate disodium using a mixed-mode column and charged aerosol detector. Liu, X.K., Fang, J.B., Cauchon, N., Zhou, P. Journal of Pharmaceutical and Biomedical Analysis , Vol. 46, No. 4, pages 639-644 (2008)
Contact me if you have questions.
Posted: Sun Sep 19, 2010 9:14 am
This material really drove me crazy.
if you want to develope a method without a high sensitivity, you can use Refractive index detector.
we have only UV and Flourscence detectors in our lab, that's why I tried to derivatize the compound with a flourogenic agent Fmoc, but the Amino group was very hard to derivatize, and the results were so poor.
I tried to undergo estrification of the hydroxyl group, with phthalic anhydride, which is a good chromogenic agent, but I needed very high concentration of the phthalic anhydride to push the reaction to completion and the remaining phthalic acid peak dwarfed the ibandronate phthalate peak and cause poor separation.
I tried to use some catalyst "pyridine" although all my colleagues were so angry about it
but even it didn't make a good job.
well, I told my boss to buy a refractive index detector for our HPLC as it was a big failure for me, and still it hurts my feelings LOOOL.
A friend of mine has a refractive index detector at his lab, and he could develop a method for it.
if you want to develope a method without a high sensitivity, you can use Refractive index detector.
we have only UV and Flourscence detectors in our lab, that's why I tried to derivatize the compound with a flourogenic agent Fmoc, but the Amino group was very hard to derivatize, and the results were so poor.
I tried to undergo estrification of the hydroxyl group, with phthalic anhydride, which is a good chromogenic agent, but I needed very high concentration of the phthalic anhydride to push the reaction to completion and the remaining phthalic acid peak dwarfed the ibandronate phthalate peak and cause poor separation.
I tried to use some catalyst "pyridine" although all my colleagues were so angry about it
but even it didn't make a good job.well, I told my boss to buy a refractive index detector for our HPLC as it was a big failure for me, and still it hurts my feelings LOOOL.
A friend of mine has a refractive index detector at his lab, and he could develop a method for it.
Re: Ibandronate sodium
Posted: Sat Oct 09, 2010 6:02 am
try C18 column with
50mm Sodium acetate and 50mm KH2PO4 with 0.5g hexane sulphonate with ph 3.5 with acetic acid and acetonitrile.
50mm Sodium acetate and 50mm KH2PO4 with 0.5g hexane sulphonate with ph 3.5 with acetic acid and acetonitrile.