Calibration curve linearity validation
Posted: Tue Mar 02, 2010 11:33 am
A question on confirmation of calibration curve verification.
In the past i have used r2 value greater than 0.999 to confirm linearity. I have received the follow statement from a cilent.
"The acceptance of the model must be verified by evaluating the relative bias between the back-calculated and nominal concentrations of the calibration standards. The use of the correlation coefficient is not recommended for confirmation of the regression model."
I am not sure how to apply this. i assume the following:
say i used nominal concenration of 2, 4, 6, 8 and 10 µg/ml stds and plot these to obtain the responses. Then re-plot with actual concentrations based on weight taken, i.e. 2.2, 4.1, 6.3, 8.1 and 10.0 µg/ml. Then compare both responses, expressed as a %.
In the past i have used r2 value greater than 0.999 to confirm linearity. I have received the follow statement from a cilent.
"The acceptance of the model must be verified by evaluating the relative bias between the back-calculated and nominal concentrations of the calibration standards. The use of the correlation coefficient is not recommended for confirmation of the regression model."
I am not sure how to apply this. i assume the following:
say i used nominal concenration of 2, 4, 6, 8 and 10 µg/ml stds and plot these to obtain the responses. Then re-plot with actual concentrations based on weight taken, i.e. 2.2, 4.1, 6.3, 8.1 and 10.0 µg/ml. Then compare both responses, expressed as a %.