Advertisement

Empower 2 and USP Resolution

Discussions about chromatography data systems, LIMS, controllers, computer issues and related topics.

2 posts Page 1 of 1
Can anyone tell me why USP Resolution is calculated using "lines drawn tangent to the peak at 50% height", but the USP plate count is determined by drawing lines tangent to the peak at 61% peak height. I think that if a peak is assumed to be Gaussian in shape, the inflection points would be at ~61%. So why the difference for resolution vs. plate count?
thanks for any help!

LaDeneVerda

Historical reasons I think. Before they made the resolution setting available to users it was at 50%. Now it can be set either way, or if you use Apex track it ignores it and used the true inflection point of the peak. Note USP's "relatively straight sides of the peak" leaves a lot of room for changes wither way.
2 posts Page 1 of 1

Who is online

In total there are 12 users online :: 0 registered, 0 hidden and 12 guests (based on users active over the past 5 minutes)
Most users ever online was 4374 on Fri Oct 03, 2025 12:41 am

Users browsing this forum: No registered users and 12 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry