Chromatography Forum

Hi Everyone.

I've got a problem with chromatograms of a PL ELSD 1000 recorded with the old software of Merck Hitachi (LaChrom): D-7000 HPLC-System Manager.

Peaks recorded never reach values above 1080 mV. I already tried to change the output from the 1/10 socket to the upper socket. But nothing changed.

I thought perhaps my solvent system itself has a to high signal. Hence I observed the baseline while changing from MeOH to MeOH/AcOH/NH4Ac but the difference was 8 mV. So that's not the issue.

Does anybody know this ELSD and if 0-1000 mV is just the detection range of that thing?

-grateful for any help. karkfeld

I'm not familiar with that particular unit, but many of the detectors that I have used allow either 10 mV or 1 V full scale.

Ok, so you mean that the maximum recording could be at 1000mV? I will check that in the manual. Did the chromatogram change in your case if you change the socket?

I checked the connections and the Detector has a 1V and a 10V output, so that's not the point...

Are you sure the "10 V" is not "10 mV" ??

yes, no doubt.

Contact Varian, who purchased Polymer Labs.

http://www.varianinc.com/cgi-bin/nav?/products/pl

It's possible that one of the voltage outputs is already integrated by the detector, whereas the other is not. The manual should clarify.