PFTBA Spectra for MRMs?
Posted: Tue Sep 23, 2025 9:30 pm
Hello everyone,
I am trying to diagnose an issue with my triple quad GC (Waters TQ-GC) and am trying to determine if there is an issue with an individual quad or the collision cell or a combination of them all. I know there are well established spectra for PFTBA single ions, but is anyone aware of established mass spectra for MRMs for PFTBA similar to what is available for single ions?
I am running an assay with some single ion scans and some MRM scans and all of the MRM scans suddenly lost their sensitivity by 99%, while only some of the single ion scans lost sensitivity by that much. In the tune, there are decent looking peaks for Q1 and Q2 but the MRMs are clearly not working. My target compounds are residual solvents, so there is a concern of my stock simply volatizing as well, but that is why established spectra for PFTBA in MRM would be so useful.
I found an Agilent paper that discussed 414>264, but would love to have more than just that one. I am aware that there are some issues with transferability between TQ instruments (my collision cell uses Ar, Agilent uses N2; different collision energies and source designs) but even so, having a list of common fragments from PFTBA would be useful.
Thanks!
I am trying to diagnose an issue with my triple quad GC (Waters TQ-GC) and am trying to determine if there is an issue with an individual quad or the collision cell or a combination of them all. I know there are well established spectra for PFTBA single ions, but is anyone aware of established mass spectra for MRMs for PFTBA similar to what is available for single ions?
I am running an assay with some single ion scans and some MRM scans and all of the MRM scans suddenly lost their sensitivity by 99%, while only some of the single ion scans lost sensitivity by that much. In the tune, there are decent looking peaks for Q1 and Q2 but the MRMs are clearly not working. My target compounds are residual solvents, so there is a concern of my stock simply volatizing as well, but that is why established spectra for PFTBA in MRM would be so useful.
I found an Agilent paper that discussed 414>264, but would love to have more than just that one. I am aware that there are some issues with transferability between TQ instruments (my collision cell uses Ar, Agilent uses N2; different collision energies and source designs) but even so, having a list of common fragments from PFTBA would be useful.
Thanks!