Hello everyone,

I’m quite new to the forum, so please excuse me if this question is not in the ideal section. I’ve been trying to understand some fundamental aspects of anion exchange chromatography, but I'm a bit stuck.

Suppose I have a 300 mM solution of phosphoric acid at pH 1.5. Since the lowest pKₐ of phosphoric acid is about 2.2, the majority of the molecules are present as neutral, fully protonated H₃PO₄. My question is: if I load this solution onto an anion exchange resin, would the neutral fraction simply pass through the column without interacting, while only the small deprotonated portion (maybe ~10%) binds?

I’d really appreciate any input or clarification on this point, and apologies again if this is too basic or not quite in the right place.