Help: Sample diluted on Injection (NanoAcquity)
Posted: Wed May 07, 2025 5:43 pm
Hey there,
We are working with a waters NanoAcquity hooked up to a Thermo Fusion lumos for detection. We are intending to inject 5 uL of sample which is in 50:50 MeOH:H2O. However, once the sample is injected, there is 15+-5 uL more liquid in the sample vial than we started with. We have tried replacing the needle and that only solves that problem temporarily.
Mobile phase A: 0.1% TEA and 1.1% HFIP in H2O.
Mobile Phase B: 50:50 MeOH:H2O.
Any insights would be appreciated, let me know if additional information would be helpful.
We are working with a waters NanoAcquity hooked up to a Thermo Fusion lumos for detection. We are intending to inject 5 uL of sample which is in 50:50 MeOH:H2O. However, once the sample is injected, there is 15+-5 uL more liquid in the sample vial than we started with. We have tried replacing the needle and that only solves that problem temporarily.
Mobile phase A: 0.1% TEA and 1.1% HFIP in H2O.
Mobile Phase B: 50:50 MeOH:H2O.
Any insights would be appreciated, let me know if additional information would be helpful.