HPLC analysis of propargyl benzoate

[natasa]

Hello. I am looking for suitable solvent for propagryl benzoate for hplc method with uv detection. I am using YMC Triart C8 and as mobile phase, water and methanol. In solvent it should be max 50% of organic compond, that I get nice peak shape. But I have problem that if I use 50 % Methanol or 50 % acetonitrile as solvent, my extraction is not high enough. I assume, that is because of hydrolyse of esther. If i use BHT in solvent, that not help. Please let me know, if you have any idea how to solve this.

[LuccasLN]

Hi Natasa,

I am very glad you are working with YMC columns and achieving good results on that! let's think of an extraction method. propargyl benzoate is hydrophobic with a stable logD of 2.2, it means you do not have to think of pH while running the extraction. I believe that the easiest way to work with that would be to run an extraction with a more apolar solvent with low boiling point, evaporate it completely and try to re-disolve it in your mobile phase. I used to do this for the analysis of vitamin E in blood plasma for example. extract Vitamin E from blood plasma with hexane, evaporate hexane completely, resuspend it in a water-MeOH mixture.

alternatively i'd try to enhance the extraction process - example: if you are working with a solid sample you might want to grind it to increase surface area or you can try temperature or US assisted extractions (then again there is the risk of degradation).

i hope this helps.

best regards,