Dear Friends,
I am working on polysaccharides molecular weight determination by GPC Pullulan standard curve. I had been using pullulan standards from shodex P82 to make standard curve however when i am using pullulan of higher molecular weight (1300kda) from agilent as part of my mix-2 iam not able to get Mw although Mp values are depicted by the calibration curve.
any feedback from the forum shall be grateful.
thanks
ashwanikumar