Chromatography Forum

Hello,

I am trying to Calibrate my GC FID 690 to Ethanol, DEE and Acetaldehyde no one has worked on liquid calibrations in my lab. Can someone help me with the calibration?

I want to know what solvents I need to use like internal standards and all. Which column would be useful?

My sample actually has Diethyl Ether, Acetaldehyde, and Ethanol.

Thank you in advance.

Do your samples consist of those three compounds dissolved in some matrix?, or are they a mixture only of those three? If they are in a matrix, what is it?

Peter

Hello Peter,

Thank you for your response. My samples contain ethanol, diethyl ether (DEE), and acetaldehyde with some water present in the product sample. I am trying to calibrate my GC-FID 690 to quantify these compounds accurately.

Could you please guide me on:

The appropriate column selection for separating ethanol, DEE, and acetaldehyde.
The best solvent choice for calibration standards.
Any specific method parameters (temperature program, carrier gas, etc.) that would be useful.
Since no one in my lab has worked on liquid calibrations before, I would really appreciate any detailed guidance.

Best regards,
Manohar

More information about the sample matrix would be helpful, but I do a direct injection of water with similar analytes (ethanol, methanol, acetone) on a WAX column. It works well.

Ethanol, Diethyl Ether, Acetaldehyde and water are the only components in my sample.
Earlier I tried to calibrate using propylene glycol as solvent and Heptanol as my internal standard but was unsuccessful.

So, I am trying to find out how to come up with a process to calibrate the GC FID to ethanol, DEE and Acetaldehyde

Manohar

What is the concentration of ethanol in your test matrices ?

Siarhei

Hello Manohar,

Has been this done ?

Why would you want to use an internal standard? You can make up external calibration standards with different proportions of your target analytes with different quantities of water, spanning from just below to just above the expected composition of your samples, and run calibration curves with those.

Peter - Manohar sounds like a raw rookie. He wrote this:

no one has worked on liquid calibrations in my lab.

So it sounds like there is no advice or mentoring available to him, sad. I was BS Chemistry, Magna cum Laude, Phi Beta Kappa, and I would've been totally lost without mentoring and learning from my co-workers and supervisors. Once HPLC became "real" and more user-friendly about 1980, we all had to learn that from the ground up.