SPME damaged after few injection

[Tania123]

I was testing the manual SPME procedure using a DVB/CAR/PDMS SPME fiber. However, after just a few injections, I noticed that the white coating was gone, leaving only a brown thin capillary visible. I wonder why this happened and what I did wrong—I don’t understand.

[rb6banjo]

Someone must have pulled the fiber apparatus out of the GC inlet or the sample vial without retracting the fiber into the protective sheath first.

You can easily strip the coating off of the end of the fiber assembly if you don't do everything in the correct order.

One other thing to check is to be sure you're using a hollow GC-inlet liner. If there is glass wool in there, it could strip your coating.

Most likely, someone didn't retract the fiber into the sheath before pulling it through a septum.