Analyte degradation during chromatographic analysis
Posted: Mon Feb 08, 2010 3:17 pm
I tested a rather structurally complex compound with 2 pPenol rings connected by an etheryl bridge: Phenyl--CH2--Oxygen--CH2--Phenyl and both phenols being chlorinated at one position each though at different carbons on the ring.
Upon chrom analysis I observed two peaks--could one be the break-away structure from the parent compound? When fractured at the --0-- site of the molecule? The Tr are at 8.5 mins and 9.3 mins. Given this veryclose association in Tr's, is this hypothesis tenable? To my knowledge, LC does not degrade compounds in situ.
Upon chrom analysis I observed two peaks--could one be the break-away structure from the parent compound? When fractured at the --0-- site of the molecule? The Tr are at 8.5 mins and 9.3 mins. Given this veryclose association in Tr's, is this hypothesis tenable? To my knowledge, LC does not degrade compounds in situ.