Hi,

I'm having difficulty finding a straightforward answer to the below question.

For each new chromatographic configuration for inlet and oven temperature is a separate RT to RI calibration curve required for analytical use when using NIST 2020 and Agilent Masshunter Qualitative Analysis Matching functions.

i.e. would we need a RT to RI calibration for each oven/inlet/column configuration or would we be able to use one for each column.

It boils down to me not knowing if each agilent datafile contains RI information in the stored data based on specified column type or only RT information.

I'm fine with the understanding of using an alkanes mixture to calibrate RT to RI but am looking for information on frequency as we budget for various calibration reference standards.

Thanks