Chromatography Forum

most of people say it is partition mechanism. but people often say adsorption of molecules on C18 rather participate into C18 phase.

My understanding is that when stationary phase is solid, then it is called adsorption chromtogram, and when is liquid, it is partition. but C18 stationary phase is liquid or solid. it is C18 liquid polymer chemically bond to silica or it is just solid? I get confused.

a

Is light a particle (photon) or a wave? Depends how you look at it. Each provides a partial description of a complex phenomenon, and each is useful in the appropriate context.

Is RPLC adsorption or partition? Depends how you look at it. Each provides a partial description of a complex phenomenon, and each is useful in the appropriate context.

in which aspect, rplc is more adsorption than partition and vise versa.

Is light a particle (photon) or a wave? Depends how you look at it. Each provides a partial description of a complex phenomenon, and each is useful in the appropriate context.

Is RPLC adsorption or partition? Depends how you look at it. Each provides a partial description of a complex phenomenon, and each is useful in the appropriate context.

At the simplest level, it doesn't matter. "Like dissolves like and like sticks to like." is a sufficient description for about 90% of what we do.

If you want a deeper understanding, read one of both of these books:
Introduction to Modern Liquid Chromatography (http://tinyurl.com/yduj7zh)
Basic HPLC and CE of Biomolecules (http://tinyurl.com/yjgpdeh)