Agilent 6475 Questions

[Ostravaczech]

In February, we got a new LCMSMS system from Agilent - 1290 and 6475, MassHunter 12.1. The system is mainly used for analysis of “compounds of potential concern, CPC” (hormones, personal care products, pharmaceuticals), and PFAS by EPA 533. Both applications are SPE enrichment, 0.5L to 1 mL for CPC, 0.25L to 1 mL for PFAS. Here are my questions:

1. Autotune only? No manual tune? I talked to the app chemist at Agilent and he says I should be able to see manual tune. I do not seem to have any options or tabs that will point me to the manual tune.

2. In the ESI negative, in order to meet MRL of 1 ppt for some of the hormones, I am at gain 10 which is the highest gain allowed while using 20 uL injection. Is this normal?

3. While using polarity switching, with gain 0.2 for ESI(+) and 10 for ESI(-), I am not able to produce decent curves, all are convex. When I split the method to 2 separate ones, the ESI(+) curves look great, ESI(-) not so much.

I sent data to Agilent this week but the app chemist I talked to really didn’t have any solutions except "clean the capillary and retune”, so I will be talking to someone else next week. In the meantime any chemist out there who has seen similar issues and may have some answers and can help?

Thank you!

[DReggio]

1. Agilent is disabling user manual tune on new equipment. It is locked behind a service account that they are not allowed to give you the password for.

It's a really unfortunate decision that has turned my off of Agilent mass specs. Their instrument will now require recalibration when previously a minor tune adjustment could keep it running.

I have not been able to get an answer from Agilent as to why they have locked users out of this essential functionality.