Retention time shift
Posted: Fri Nov 03, 2023 4:48 pm
Hello,
I have been using a Dionex ics-5000+ since may and have had no problems until recently the peaks I am analyzing have shifted about 1.0-1.2 minutes. The total run time is 5.0 min with a normal retention time of 1.8-2.3 min and 2.6-3.1 min. The flowrate set is 1.6 mL/min, column temp at 35 degrees. I have changed out the column and suppressor prepped fresh mobile phase (30 mM MSA) and am still having this issue. My system suit fails every run because of the resolution between peaks. Is there anything I can try out to fix this problem?
I have been using a Dionex ics-5000+ since may and have had no problems until recently the peaks I am analyzing have shifted about 1.0-1.2 minutes. The total run time is 5.0 min with a normal retention time of 1.8-2.3 min and 2.6-3.1 min. The flowrate set is 1.6 mL/min, column temp at 35 degrees. I have changed out the column and suppressor prepped fresh mobile phase (30 mM MSA) and am still having this issue. My system suit fails every run because of the resolution between peaks. Is there anything I can try out to fix this problem?