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Negative peaks

http://www.chromforum.org/viewtopic.php?t=1216

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Negative peaks

Posted: Wed Dec 22, 2004 1:37 am
by sarex
Can anyone advise on why we get negative peaks on our solvent front with a reverse phase assay method when the sample solvent is mobile phase. Wavelength is 210nm and mobile phase is 0.4% THF, 1.0% Isopropyl Alcohol, 98.6% water plus TFA to pH 2.6
Is it the TFA that causes this problem ?.

Any hints would be appreciated. Thanks.

Sarex

Those Darn Negative Peaks

Posted: Wed Dec 22, 2004 4:46 pm
by GoldLeader
Sarex-
Log in to www.waters.com. On their home page search window type " HPLC Troubleshooting". The second result that comes up will be for their Information Center for a publication by Dr. Uwe Neue (#WA 20769). You can download the whole thing in pdf. There is a chapter in there taht describes your situation very closely. Hope it helps.
-Gold Leader

btw- I'm not associated with Waters in any way...
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