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Capacity factor USP 621 (error)

Posted: Thu Jan 28, 2010 12:50 pm
by Rivka Goldik
Hi all:
I found in the USP general chapter about chromatography twice (at the GC and HPLC titles) that the capacity factor k' depends on flow rate of the mobile phase. I think that this statement is wrong . What do you think?

Posted: Thu Jan 28, 2010 3:18 pm
by HW Mueller
The flow rate would cancel out:

k´= (tr - tm)/tm (tm is also written as to)