GPC of dextrins: tailing problems

[filmstar]

Hello, everyone!

I am running alpha amylase treated dextrins (mixtures of simple sugars and oligosacchrides) on Superdex 30, but now have severe tailing problems with my samples.

When I run the standards, e.g. glcose, there is no tailing.
but when I ran dextrins, the tailing become very long. even i reduced the injection volume, still the tailing is there.

I used 0,05M NaCl in water as eluent.

Anyone has any idea please kindly help me.

[tom jupille]

Are you seeing tailing that extends past the total permeation time? If not, you may just have a skewed molecular size distribution.