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- Posts: 11
- Joined: Wed Dec 30, 2009 4:49 pm
Hi all
I'm analysing lipids in (really) fatty tissues. To do it, i do a first lipid extraction using chloroform. As lipids are not soluble in acetonitrile:water mixtures I need to dissolve them in chloroform solutions.
Now the point is.. what's next? Is is possible to do a RP-UPLC-MS analysis using a methanol:chlroroform solution as sample solvent or that will destroy the columns and split the peaks?...
Maybe it'd be better to derivatize and use GC-MS??
I'm analysing lipids in (really) fatty tissues. To do it, i do a first lipid extraction using chloroform. As lipids are not soluble in acetonitrile:water mixtures I need to dissolve them in chloroform solutions.
Now the point is.. what's next? Is is possible to do a RP-UPLC-MS analysis using a methanol:chlroroform solution as sample solvent or that will destroy the columns and split the peaks?...
Maybe it'd be better to derivatize and use GC-MS??
