Chromatography Forum

why phosphate buffer become the choice of buffer for RPLC?
uv cut off should be one reason, do you know any other reasons?

for starters, phosphate buffers (mono- and dibasic at least) have very good buffer ranges for HPLC column pH limitations.

Secondly, for UV detectors, they have no chromophores that will interfere with that detector.

However having said that, phosphate buffer is non-volatile, therefore not to be used with an ELSD or a MS detector.

Cost and its pervasive use in the world of LC are other factors.

hi, i am psu alumni too.
thanks for your answer,
is there any reason related to prevent ion exchange mechanism for this buffer.

for starters, phosphate buffers (mono- and dibasic at least) have very good buffer ranges for HPLC column pH limitations.

Secondly, for UV detectors, they have no chromophores that will interfere with that detector.

However having said that, phosphate buffer is non-volatile, therefore not to be used with an ELSD or a MS detector.

Cost and its pervasive use in the world of LC are other factors.

is there any reason related to prevent ion exchange mechanism for this buffer.

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Phosphate the new TFA? Not sure about that, but it could offer some selectivity differences. What are you analyzing?