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anyone use Hypercarb Columns before

http://www.chromforum.org/viewtopic.php?t=12072

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anyone use Hypercarb Columns before

Posted: Wed Jan 20, 2010 6:21 am
by jiang295
is there any serious irreversible adsorption?

Posted: Wed Jan 20, 2010 4:51 pm
by skunked_once
This is a question that is difficult to answer because for almost any column, there may be some type of sample that could irreversibly bind. It would help if you could post what type of samples you expected to use with the column.

Posted: Thu Jan 21, 2010 5:54 am
by Kostas Petritis
You will get serious adsorption for polyaromatic compounds. THF is a good solvent for cleaning these columns. Negative charged molecules may also interact with this type of column under some conditions...
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