Chromatography Forum

i am new to this software and a few things are not exactly intuitive....

For example:
How do you change the "path" in the "Sequence Parameters" window?

And does anyone out there have a post run macro for an Agilent 1100 that shuts down everything but the cooler tray in the auto-loader module?

Thanks
Dirk

i am new to this software and a few things are not exactly intuitive....

For example:
How do you change the "path" in the "Sequence Parameters" window?

Disclaimer: I'm only experienced with A.10 and earlier ChemStations.
The default path for your Intrument 1 is C:\HPCHEM\1\DATA\ then you enter in the next subdirectory, like 1-14-10\ and your data goes in there.
For your Intrument 2 it's C:\HPCHEM\2\DATA\ then you enter in the next subdirectory, like 1-14-10\ and your data goes in there.

Now the C:\HPCHEM\1\DATA\ part is grayed out. If you want to change that, that's in the [PCS] section of the win.ini file, and you can change it there to whereever you want.

From your comment of Agilent1100 I assume you are using LC-chemstation.

The sequence parameters window allows you to choose a path from a list of paths you have defined (at least in Revision B). To define them, Revision B onwards, stay in the Method and Run view, and select "View" - "Preferences" from the menus at the top of the screen. This brings up the data and method file paths, with "add" and "remove" buttons so you can add a new path. This will then become available on the Sequence Parameter page.

In older versions of Chemstation, the easiest way to change the path is to close chemstation, open the Configuration Editor, select "Paths", choose your new path, save the configuration, and exit, and re-open chemstation. There is a way to do it from the command line at the bottom of the screen, but I can't remember the thing to type.

On our Agilent 1100 system, if you run a sequence with "STANDBY" selected in the post-sequence macro place, then it turns off the pumps and lights in the DAD, but it leaves the autosampler thermostat on cold, so if yours is the same, you don't need a macro.

Thanks, I'll give the standby command a try.

And the configuration editor did the trick.

Thanks IMH

On our Agilent 1100 system, if you run a sequence with "STANDBY" selected in the post-sequence macro place, then it turns off the pumps and lights in the DAD, but it leaves the autosampler thermostat on cold, so if yours is the same, you don't need a macro.

For some reason our autosampler thermostat sets to ambient instead of cold. Is there somewhere to configure "standby" or a setting that needs to be toggled to the "on" position?

Thanks

On our Agilent 1100 system, if you run a sequence with "STANDBY" selected in the post-sequence macro place, then it turns off the pumps and lights in the DAD, but it leaves the autosampler thermostat on cold, so if yours is the same, you don't need a macro.

For some reason our autosampler thermostat sets to ambient instead of cold. Is there somewhere to configure "standby" or a setting that needs to be toggled to the "on" position?

Thanks

For that reason I use own macro:
http://rapidshare.com/files/350865697/SHUTDOWN.MAC

Save this file to your Chemstation\Core directory. Then set Post-sequence Cmd / Macro in sequence parameters in Shutdown section to "macro "SHUTDOWN.MAC", go

Hope it helps.
Regards

Thanks, I'll give it a try.

you're quite right, it ought to turn the autosampler thermostat off (according to the helpfile), but ours clearly doesn't turn off! Strange...

I suspect your macro needs only two lines; PumpAll off and LampAll off. If you have an MS, you may need MSSetState STBY. Does anyone know if these can be included in the sequence parameter box, or whether they have to be in a macro because there's more than one command?

Name shutdown
PumpAll off
LampAll off
EndMacro

Is that should be all you need for a shutdown.mac that will leave my samples at 4°C but turn off the pumps and lamp?

Is there any special syntax or format required?