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How to make calibrate curve?

Posted: Wed Sep 13, 2023 5:34 am
by itsarapan
Hello everyone; I’m quite new in chromatography. I have the experiment to quantify the metabolite concentration in my tissue sample. I have to make a calibrate curve, but I don't know how much to adjust the appropiate concentration of the lowest to the highest dilution to make a calibrate curve. Please give me some advices.

Thank you so much