Agilent colums
Posted: Wed May 24, 2023 9:21 am
by Saba Nazir
May any one tell me that why hump is coming g in the chromatogram of GCMS with J&W DB-5MS column. Column is new and conditioning g has been preformed?
Re: Agilent colums
Posted: Wed May 24, 2023 9:01 pm
by James_Ball
Is this a rise in baseline at the end of the chromatogram on a temperature programmed run or is it in the middle of the chromatogram?
Also what is the dominate mass present in the spectra of the hump you are seeing?
Re: Agilent colums
Posted: Wed Aug 02, 2023 8:05 am
by Da_Li
Hello Saba,
I agreed with James, it's depending on where are you get the hump. Normally the column bleed would occur with raising temperature, you could have a look on the oven temperature.
Then, the new o-rings and septum is an potential source of the hump as well.
Basically, mass on 207 (major), 281, and 73 are shown for the column bleed.
Hope this would be useful on your case.