method detection limit
Posted: Mon Jan 11, 2010 8:43 pm
A question to the method detection limits.
I am working on an urine method detection limits. Dilute the urine sample 30 times to approaching the detection limits. Analyze 10 replicate of the diluted sample. Calculate the STDEV. Then the detection limit will be the 2.82 X STDEV. (MDL=0.02)
But for the real sample, I will dilute the sample 5 times. My questions it that for the detection limit, do you need to multiply the dilution factor? Should my method detection limit be 0.02X30=0.6 or 0.02 X5 = 0.1?
I am working on an urine method detection limits. Dilute the urine sample 30 times to approaching the detection limits. Analyze 10 replicate of the diluted sample. Calculate the STDEV. Then the detection limit will be the 2.82 X STDEV. (MDL=0.02)
But for the real sample, I will dilute the sample 5 times. My questions it that for the detection limit, do you need to multiply the dilution factor? Should my method detection limit be 0.02X30=0.6 or 0.02 X5 = 0.1?