Enzymatic reactions - workup for GC analysis?
Posted: Fri Mar 03, 2023 9:20 pm
Not at all familiar with this type of sample. I have a student who runs enzymatic reactions (amine couplings of small molecules) using the enzymatic activity of things like yeasts and molds (candida, aspergillus, Lipase-B, etc.) and they would like to perform analysis by GC/FID and GC/MS.
I'm reading that typically centrifugation is used in order to purify the yeasts and enzymes, but is centrifugation a good enough method to get everything out of the mixture to be analyzed? Would the enzymes produced by the yeasts also be taken out like the yeasts/molds?
Just want to avoid anything that could be detrimental to our instruments or columns. Most of the stuff I end up finding if the opposite of what I'm looking for (looking at the yeasts/enzymes, not the reaction they catalyzed). If we are not interested in those components, but only in the small organic molecules, is simple filtration over silica enough?
I'm reading that typically centrifugation is used in order to purify the yeasts and enzymes, but is centrifugation a good enough method to get everything out of the mixture to be analyzed? Would the enzymes produced by the yeasts also be taken out like the yeasts/molds?
Just want to avoid anything that could be detrimental to our instruments or columns. Most of the stuff I end up finding if the opposite of what I'm looking for (looking at the yeasts/enzymes, not the reaction they catalyzed). If we are not interested in those components, but only in the small organic molecules, is simple filtration over silica enough?