Chromatography Forum

because it is very hydrophilic?

It is good for the SEC of proteins and peptides, which contain multiple charges. It also binds water rather well, and has close to no hydrophobicity. With silica-based packings, these properties have been emulated with well-designed diol packings.

can you make sure that there is almost no hydrophobic interaction between it and rather hydrophobic organic compounds?

It is good for the SEC of proteins and peptides, which contain multiple charges. It also binds water rather well, and has close to no hydrophobicity. With silica-based packings, these properties have been emulated with well-designed diol packings.

Dextran was first used (and still is) as plasma substitute. Hence, it was known that Dextran has low irreversible interaction with proteins. When locking for a material suitable as stationary phase for GPC of proteins and pepetides, Dextran was the first (and lucky) choice.

how about small molecules rather than protein?

Dextran was first used (and still is) as plasma substitute. Hence, it was known that Dextran has low irreversible interaction with proteins. When locking for a material suitable as stationary phase for GPC of proteins and pepetides, Dextran was the first (and lucky) choice.

At 1:11 am in the other chain, I already tried to do something to get on with this question. If you don´t reply to that I can´t help you further.