Chromatography Forum

Hello All, it's me again.

And I have another Chromeleon question. This time about integration of SEC chromatograms.
I run a separation of proteins and peptides on a TSK Biogel column. The result is your typical SEC chrom. A range of overlapping peaks and plateaus, which is no problem, because that is what I expect.
The question is about integration of this. I'm interested in the Area% of a couple of molecular weight ranges. Through a set of standards I have calculated what the Rt's are of different mw's like 10KDa, 5KDa etc. And I want to know the Area% of let's say from 10KDa to 5KDa.
What I do now is manually split the peaks at specific times for the whol chromatogram, but I want to know if there is a way to do this automatically? Which integration parameters should I use to do this?

Thanks again for your time and happy Holidays!
Maurice

Maurice,

if you have a dinstinct chromatographic featue of your peaks like a valley or a peak shoulder, then you can automate the process which will allow for peak recognition.

if you have a broad polymer distirbution whihc give you simply a broad peak in the chromatogram thne it will probably need to be done manually.
the possible parameters to use will mainly be:

peak slice
sensitivity
peak shoulder
be aware that they are interrelated parameters. they work all together. the peak shoulder is especially influenced by the sensitivity.
use the help files (F1) in order to better understand how it works. there are tips there to help you.

Check out the help file topic for Left/Right Limit (by default they are hidden columns on the peak table tab of the QNT)