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Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

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TimB
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Last edited by TimB on Tue Sep 14, 2010 8:50 pm, edited 1 time in total.

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HW Mueller
In my work it has not been unusual to have some matrix elements which do not chromatograph correctly under the chosen conditions. If these "humps" are interfering you will have to do some precleaning or multi-step chromatography.

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MHD
Hi Tim,

Could you tell me the sample solvent you used, always we used the mobile phase as sample solvent. (For using a absolutely organic phase as sample solvent can cause this phenomenon.)
You can try to use less injetion volume to see wether the hump before the main peak is still exist.
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