Chromatography Forum

Our lab would like to purchase a refractive index detector for carbohydrate analysis. Has anyone had any positive or negative comments on the Shodex 101 or Shimadzu RID-10A ?
Any thoughts or suggestions with refractive index technology only would be appreciated.

Also, could you please suggest a good analytical column using an RI detector for Sucrose, Fructose, & Glucose. I'm looking at about 500 injections per week for routine analysis. Thanks...

Although I am very bias, I have also included a very easy procedure for our specialty design column (VA 300/7.7 NUCLEOGEL SUGAR 810 Ca ) to separate sugar.

Here is the procedure https://www.macherey-nagel.ch/web/MN-WE ... WIK-7XQFUR

I hope you will pick our product.

Also, could you please suggest a good analytical column using an RI detector for Sucrose, Fructose, & Glucose. I'm looking at about 500 injections per week for routine analysis. Thanks...

BioRad (among others) also makes several columns for sugars. I'd search the vendor sites for a chromatogram featuring good seperation of your analytes in a short time using a fairly simple mobile phase. As far as RI detectors fo, I have experience w/ Waters. It's decent. AFAIK, all RI detectors take a while to come to thermal equilibrium - and this is critical to their baseline stability. Based on that, questions to ask vendors would likely include "How long does your RI take to equilibrate? How sensitive is it to external temperature fluctuations? What's the signal to noise ratio? What's its useful range?"

I use a BioRad column, Aminex #HPX-87C, with deashing precolumns (to remove excess cations and anions) to separate lactose, glucose and galactose. My RiD is from Agilent, and I turn on my HPLC system the afternoon before I plan to inject, to give the system adequate time to equilibrate.

I run very infrequently, so I cannot give you any information regarding the lifetime of the column. I can tell you that BioRad is an excellent resource, and you will probably want one of their Aminex columns. I looked at other column manufacturers, but their literature was too labyrinthine.

Good luck!

Anne,

There are many columns and vendors for carbohydrate analysis columns. If you would like to see the choices my company offers, please go to the chromatography section of our website, and you can download a copy of our catalog. There are several examples of this separation. For such a simple analysis, you may want to consider a sodium form or mixed mode type column, rather than 100% calcium loaded. You will get a faster analysis, easy to regenerate (sodium form), and in my experience, longer lasting sharp peak for glucose. Good luck in your search.

http://www.transgenomic.com/

Bart

A simple procedure is the analysis of these sugars on an amino-type column. The Carbohydrate Analysis Column from Waters has been specifically prepared to give you a stable analysis of sugars.

I confirm what Uwe said, Waters sugards columns are very stable and robust.

Why don't you develop for the future needs.

If you go for HILIC already now you will use a highly volatile mobile phase suitable for ELSD (and later when the price is nice a MS detector)

Thanks for taking the time to respond to my e-mail, I really appreciate all those who responded. This has helped me out. Thanks.

Anne

Hello all:

You can go two ways in picking up the column.

1- Use acetonitrile/water as mobile phase. You will need an amino columun and the anlysis can be done at room temperature. Also the solvents are toxic.

2- Use Water as mobile phase. In this case you will need and column similar to the ones discussed above such as the Biorad. Have in mind that this anlisis is done at temperatures above 70 centigrades, so you will need an oven.

Regarding the detector. I have used the Shimadzu detector with out any problem in a high productivity envioroment. Anyhow take in consideration the service suport avilable in your area. Also it would be a good idea to purchase an aditional lamp if you will be using it a lot.

We developed a method last year for the sugars you have listed. We tried out both the Waters Carbohydrate amino column with a AcN/water MP and an IEX type with pure water. We found that one could obtain significantly higher S/N with the pure water MP. Has anyone else observed this with RID?