alternative way to column chromatography ???
Posted: Mon Dec 14, 2009 2:34 pm
Dear,
We use column chromatography method for fractination of saturated and aromatic hydrocarbons of petroleum samples. Firstly We prepare the column with activated silicagel-alumina. Sample moves down on to alumina, elute with solvent. etc...After seperation, we collect saturated and aromatic hydrocarbons seperately and do GC-MS analysis..But it takes too long time.
We need an alternative method. Is there any effective HPLC for this purpose?
We use column chromatography method for fractination of saturated and aromatic hydrocarbons of petroleum samples. Firstly We prepare the column with activated silicagel-alumina. Sample moves down on to alumina, elute with solvent. etc...After seperation, we collect saturated and aromatic hydrocarbons seperately and do GC-MS analysis..But it takes too long time.
We need an alternative method. Is there any effective HPLC for this purpose?