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Peak Tailing Issue

http://www.chromforum.org/viewtopic.php?t=118654

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Peak Tailing Issue

Posted: Thu Sep 15, 2022 3:57 pm
by laghates
Hi,

I am currently working on a HPLC method. It is an isocratic method, which uses DI water as mobile phase, RI detector for detection and Aminex HPX 87C (7.8 x 300 mm) column. The flow rate is 0.6 mL/min and column temperature is 65C. I am seeing tailing peak for my sample, which the column standard is coming out great. How can I fix tailing on my sample peak?

Re: Peak Tailing Issue

Posted: Thu Sep 15, 2022 5:39 pm
by Steve Reimer
Does you sample match the mobile phase? What is the injection volume?

Re: Peak Tailing Issue

Posted: Thu Sep 29, 2022 2:51 pm
by laghates
gg

Re: Peak Tailing Issue

Posted: Thu Sep 29, 2022 2:54 pm
by laghates
Does you sample match the mobile phase? What is the injection volume?
Yes the sample is dissolved in the Mobile phase (DI Water). I am loading 200 ug on the column.
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