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PDA Peak extraction
Posted: Wed Aug 17, 2022 4:31 pm
by Mouleyre
If you run a sample and see multiple peaks using LC-UV and want to extract one of the peaks for PDA analysis, how would you do that?
Re: PDA Peak extraction
Posted: Thu Aug 18, 2022 8:51 am
by wss
Collect the peak as it elutes out of the detector and then concentrate/reinject? That's what we used to do for peptides in my old organic lab. Collect the fraction in an Eppendorf tube and then walk it over to the mass spec.
What do you mean by PDA analysis?
Re: PDA Peak extraction
Posted: Thu Aug 18, 2022 1:55 pm
by DR
If you're interested in obtaining a UV spectrum for a particular peak via extraction of PDA data, that is doable, but again - more info needed.
a PDA can collect a range of wavelengths from which people choose to extract either an entire chromatogram at particular wavelengths, or they want the whole spectrum for particular peaks. Both are pretty easily done, provided the PDA was setup properly for the collection part.