Regenerate HPLC colum

[camino]

Hello,
I'm using a C18 LiChrocart and abruptly the pressure is down, and the resolution of the peaks is very poor. I think that I need to regenerate the column. What I need to do?

I think that the steps I need do are: Wash with water, then methanol, and finally with chloroform, and then reverse in the order of the wash solvets.

Are this ok???

Thank

[MG]

The pressure is abruptly lower? Are you sure you don't have a leak somewhere in your system?

[Black Sunshine]

If your retention times have also shifted (earlier), there is a possibility that the packing of your column is shot and/or channneling.

[blackdrum]

Maybe regeneration is useless, you should refill the column head, but it's difficult to do well