Extracting a peak for UV/VIS Analysis

[Mouleyre]

Does anyone know what is entailed in extracting a peak from a HPLC-UV chromatogram for UV-VIS analysis?

[DR]

Not likely based on the information you've not provided...

Please add some specifics, like what data system you're using for starters.

[Mouleyre]

Maybe this query will make more sense:

If you run a sample and see multiple peaks using LC-UV and want to extract one of the peaks for PDA analysis, how would you do that?

[sbashkyrtsev]

When working with PDA, you probably want to extract a wavelength chromatogram. If only one substance absorbs well on that particular wavelength, then you'll eventually have an extracted chromatogram with just one peak. But if other compounds also absorb at that wavelength, you'll still end up with multiple peaks.

[Mouleyre]

When working with PDA, you probably want to extract a wavelength chromatogram. If only one substance absorbs well on that particular wavelength, then you'll eventually have an extracted chromatogram with just one peak. But if other compounds also absorb at that wavelength, you'll still end up with multiple peaks.

Please give a step by step on how to do this with lets say a LC-UV chromatogram with 2 peaks.

[vmu]

Please give a step by step on how to do this with lets say a LC-UV chromatogram with 2 peaks.

1. Find the corresponding command for the chromatogram extraction in your software.
2. Choose the wavelength you need (from the recorded range of wavelengths) and use this command.