Advertisement

different retention times on repeat runs of the same sample

Discussions about GC and other "gas phase" separation techniques.

2 posts Page 1 of 1
I am an newbe at GC so please bear with me.
Here at work we have an older GC with a packed column and a FID detector that I am trying to use to quantify some additives and contaminates in our mill rolling oil. The peaks I am having trouble with are from an additive - lauric acid. I see the peak fine, but the retention time isn't always the same. For the duplicates of the sample I was running today, the peaks were sometimes 10 sec apart which is playing havoc with my peak analysis efforts.

I inject the sample manually, but I know I have less than 1 second variation in my injection technique - certainly not 10 seconds.

My heating profile lets it set for 5 minutes then it ramps up evenly to max temp over the next 25 minutes. Is this something I should change? Or is there somewhere else I should be looking to solve this problem?

Could some of you more experienced folks give me some ideas on how to reduce the variation in retention time?

Thanks,
Ron

what solvent is it in, injection volume, and flow rate? what are some of the other analytes in the mix? are there any other retention time problems (ie, are the other peaks eluting consistently?)
2 posts Page 1 of 1

Who is online

In total there are 82 users online :: 0 registered, 0 hidden and 82 guests (based on users active over the past 5 minutes)
Most users ever online was 5108 on Wed Nov 05, 2025 8:51 pm

Users browsing this forum: No registered users and 82 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry