Vitamin A Palmitate Powder Form

[dude]

I have a problem in extracting Vitamin A palmitate in oral solution, i try saponification process but it didnt work. The Vit. A palmitate is not soluble in methanol, acetonitrile but i succeded in using NaOH solution. Can you help me in finding other method in this situation.

[Consumer Products Guy]

I have a problem in extracting Vitamin A palmitate in oral solution, i try saponification process but it didnt work. The Vit. A palmitate is not soluble in methanol, acetonitrile but i succeded in using NaOH solution. Can you help me in finding other method in this situation.

Dude - try THF.

That's the first time I've ever used the word "Dude"....

[dude]

Thanks! but it didnt work also because it is insolube in THF.

[HW Mueller]

Depends on what you mean with "soluble". Curiosity led me to just google: it is somewhat soluble in EtOH, usually it seems to be formulated in oils. If it dissolves in oils it should be reasonably soluble in hydrocarbons.
I also wonder about your inability to solubilize via saponoification, but NaOH did this??

[Consumer Products Guy]

Thanks! but it didnt work also because it is insolube in THF.

I'll try to look up what we've used in the past for A palmitate when I return from vacation Friday. Memory had it that it was tHF, but you seem to say otherwise. Maybe it's a matter of concentration????

[aceto_81]

Maybe your vitamine is coated, try to peel it off with diluted HCl

Ace

[danko]

Is it the plan to analyse it by means of chromatography? And if so, do you have a method for that? If still so, what is the utilized mobile phase?

I would expect that the compound is soluble in the mobile phase

Best Regards

[Consumer Products Guy]

OK, I checked. Initially ee dissolved 0.2g vitamin A palmitate (from Roche) in 100 ml DMF, then diluted down further. HPLC was on RP-8 using H2O-THF.

[Bruce Hamilton]

Vitamin A Powder Form used to be a dispersion in gelatin or acacia.

In my experience, you really need to be very careful during extraction ( using actinic glassware etc., and use antioxidants like BHT ) to ensure the molecules stay intact. I think the BP or EP detailed the precautions for the powder form, and it's important to use a very good grade of IPA.

HPLC was by C8 with 20:1 methanol: water. I recall that Roche had useful analytical advice information freely available, perhaps still available, if you ask nicely.

Please keep having fun,

Bruce Hamilton

[dude]

thanks to you all im going to try your suggested method.