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Ultimate3000 DAD detector error "Signal Overload Detected"
Posted: Mon Jul 04, 2022 6:48 am
by bunkaAK1
Hi All,
I am having a problem with the DAD detector on Ultimaet 3000. It came up with the error: "signal overload detected". Does anyone know what it means or what causes the error?
Thanks
Re: Ultimate3000 DAD detector error "Signal Overload Detected"
Posted: Mon Jul 04, 2022 7:15 pm
by vmu
Reduce the injected amount either by reducing the sample concentration or by reducing the injection volume.
Re: Ultimate3000 DAD detector error "Signal Overload Detected"
Posted: Tue Jul 05, 2022 8:49 am
by bunnahabhain
I had similar trouble when using a weak lamp together with eluent containing formic acid. So try to flush your flowcell with pure water before turning on the lamp. If this works and the problem comes back with your eluent of choice, maybe a new lamp will help, but you should consider switching to another eluent additive.
Good luck
Jörg
Re: Ultimate3000 DAD detector error "Signal Overload Detected"
Posted: Wed Jul 13, 2022 5:30 am
by bunkaAK1
Thank you for the advice!