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Vitamin A Palmitate Powder Form
Posted: Wed Nov 18, 2009 7:22 am
by dude
I have a problem in extracting Vitamin A palmitate in oral solution, i try saponification process but it didnt work. The Vit. A palmitate is not soluble in methanol, acetonitrile but i succeded in using NaOH solution. Can you help me in finding other method in this situation.
Re: Vitamin A Palmitate Powder Form
Posted: Wed Nov 18, 2009 2:45 pm
by Consumer Products Guy
I have a problem in extracting Vitamin A palmitate in oral solution, i try saponification process but it didnt work. The Vit. A palmitate is not soluble in methanol, acetonitrile but i succeded in using NaOH solution. Can you help me in finding other method in this situation.
Dude - try THF.
That's the first time I've ever used the word "Dude"....
Posted: Thu Nov 19, 2009 12:33 am
by dude
Thanks! but it didnt work also because it is insolube in THF.
Posted: Thu Nov 19, 2009 9:36 am
by HW Mueller
Depends on what you mean with "soluble". Curiosity led me to just google: it is somewhat soluble in EtOH, usually it seems to be formulated in oils. If it dissolves in oils it should be reasonably soluble in hydrocarbons.
I also wonder about your inability to solubilize via saponoification, but NaOH did this??
Posted: Thu Nov 19, 2009 11:22 pm
by Consumer Products Guy
Thanks! but it didnt work also because it is insolube in THF.
I'll try to look up what we've used in the past for A palmitate when I return from vacation Friday. Memory had it that it was tHF, but you seem to say otherwise. Maybe it's a matter of concentration????
Posted: Fri Nov 20, 2009 8:45 am
by aceto_81
Maybe your vitamine is coated, try to peel it off with diluted HCl
Ace
Posted: Fri Nov 20, 2009 9:47 am
by danko
Is it the plan to analyse it by means of chromatography? And if so, do you have a method for that? If still so, what is the utilized mobile phase?
I would expect that the compound is soluble in the mobile phase
Best Regards
Posted: Fri Nov 20, 2009 2:58 pm
by Consumer Products Guy
OK, I checked. Initially ee dissolved 0.2g vitamin A palmitate (from Roche) in 100 ml DMF, then diluted down further. HPLC was on RP-8 using H2O-THF.
Posted: Fri Nov 20, 2009 8:16 pm
by Bruce Hamilton
Vitamin A Powder Form used to be a dispersion in gelatin or acacia.
In my experience, you really need to be very careful during extraction ( using actinic glassware etc., and use antioxidants like BHT ) to ensure the molecules stay intact. I think the BP or EP detailed the precautions for the powder form, and it's important to use a very good grade of IPA.
HPLC was by C8 with 20:1 methanol: water. I recall that Roche had useful analytical advice information freely available, perhaps still available, if you ask nicely.
Please keep having fun,
Bruce Hamilton
Posted: Wed Nov 25, 2009 4:36 am
by dude
thanks to you all im going to try your suggested method.