Waters 2796 Bioseparations HPLC

[ChromatographyDude]

Hello,

Hoping someone can provide me with some quick suggestions. I am trying to help a sister company get up and running with their Waters 2796 HPLC system. I have worked with other HPLC systems in the past, not this particular brand.

How do I determine what size syringe is installed in the system? And how about the sample loop volume?

I've noticed that the vacuum degasser is having issues. It stays on for a few minutes into a run, and then it shuts off and gives me an error message. Is there any maintenance I can do on this degasser?

Finally, what is the best approach to ensure that my injection is actually taking place? I think the autosampler is very unusual, I simply put in a tray into 1 of 4 slots, and it doesn't even fit in securely. But evidently it appears to be injecting.

Thanks for any help.

[ChromatographyDude]

Does the degasser need to be connected to an external gas supply? It was only reading around 1psi prior to shutdown. What is an acceptable pressure? Thanks!!

[ChromatographyDude]

The degasser error message is: h/w fault (3). The problem seems to be intermittent with the degasser turning off at different times throughout the day. Thanks for any feedback!

[Mattias]

You will need to contact Waters regarding the degasser, this is not something that I would try to fix myself. It does not require any gas.

The syringe is in the front of the instrument, quite hard to miss actually. To change the loop you need to remove the column oven.

The actual syringe and loop size must entered in the Config menu of the Alliance system - you can check there what has been entered.